上海交通大学生物化学2003年度Test Paper1※2及答案

上海交通大学生物化学试卷(2003 年度)



2003 TEST PAPER (1)(闭卷部分)
(Shanghai Jiao Tong University,1st term of Univ. year 2003) Subject: Biochemistry Name _____________ Class _____________ Score _____________

一. Explain the terms:(2.5 x 10= 25)
1. hyperuricemia

2. LDL receptor

3. pyruvate dehydrogenase complex

4. glucokinase (in liver)

5. malate-aspartate shuttle


7. substrate cycle

8. ―macro CoA‖

9. urea cycle

10. UDP- glucose

2003 TEST PAPER (2)(开卷)
(Shanghai Jiao Tong University,1st term of Univ. year 2003) Subject: Biochemistry Name _____________ Class _____________ Score _____________

二. Filling the blanks: (2.5 x 12= 30) 1. Many important coenzymes, such as____, ____and ____, are the derivatives of ADP; dNDP are synthesized from ____ and dTMP is formed by________ of dUMP; all these facts reflects that_____ came before______ in evolution. 2. TPP is the derivative of vitamin ____, it serves as the prosthetic group of the following enzymes: _____________, _____________and_____________. 3. There is a large difference between the yield from the complete oxidation of triacylglycerols and of carbohydrates (and protein), the reason is_________________________. 4. The three pairs of enzymes that catalyze the three resemble steps in β-oxidation of FA and citric acid cycle are____________ and_____________, _____________and_____________, _____________and_____________. 5. The only reactions in mammals that require coenzyme B12 are__________ and _______________________ catalyzed by ________ and _____________, respectively. 6. OAA has several possible fates: _____________, ________, _____________ and _____________. 7. In the synthesis of phosphoglycerides 磷酸甘油酯 and of glycogen, the activated intermediates are _____________ and____________, both of them are formed from a _____________ substrate and a _____________, and then react with a _____________ group. 8. The committed step in cholesterol biosynthesis is the synthesis of __________ from _____________which is catalyzed by _____________. 9. The carrier of the activated one-carbon units is_____________ which consists of three groups: ____________, ________ and_____________. Besides, _____________ is the major donor of methyl group. The fully oxidized one-carbon unit is _____________, which is carried by_____________. 10. Disease and inherited defects of enzyme or functional protein, e.g. PKU—Phe hydroxylase; drug-induced hemolytic anemia 溶血性贫血—_____________; hypercholesterolemia 高胆 固醇血症—_____________; pernicious anemia 恶性贫血—_____________; maple syrup 槭糖浆 urine disease—_____________; Leber’s optic neuropathy 视神经症 —_____________. 11. Acetyl CoA carboxylase from E. coli consists of three subunits called __________, _______ and ______. 12. There are two types of covalent modification: _____________ and_____________in the regulation of enzymatic activity, the typical example of which is_____________ and _____________(enzyme), the direct related residue is______ and ______, respectively.

三.Make choice (one or several):(3 x 8= 24) 1. Schiff base intermediates are formed in the reactions catalyzed by
A. aldolase B. glycogen phosphorylase aminotransferase C. Trp synthetase D. transaldolase E.


3. 4.





Multifunctional (or bifunctional) enzyme occurs in A. pyrimidine synthesis of eukaryotes b. FA synthesis of eukaryotes C. the synthesis and hydrolysis of F-2,6-BP D. the phosphorylation and dephosphorylation of isocitrate DH E. FA synthesis of E. coli PRPP participates in the synthesis of the following substance A. Pro B. His C. purine D. Phe E. pyrimidine Biotin, as the posthetic group of the following enzyme A. acetyl CoA carboxylase B. transketolase C. pyruvate carboxylase D. Pyr DH E. α–KG DH The following complex contains Fe-S cluster A. NADH-Q reductase B. cytochrome c reductase C. cytochrome c oxidase D. succinate-Q reductase E. ribonucleotide reductase Which substance is the direct precursor of the synthesis of acetoacetate 乙酰 乙酸 in liver A. HMG CoA B. acetoacetyl CoA C. D-3-hydroxybutyrate 羟丁酸 D. D-3-hydroxybutyryl CoA E. acetone Citric acid cycle A. it can produce high-energy phosphoryl compound by itself B. it is not inhibited by the anaerobic 厌氧 condition C. the two carbon atoms of acetyl CoA are liberated as the form of two CO2 in that round of the cycle D. its rate depends upon the need of ATP E. it is the final common pathway for the oxidation of fuel molecules. It participates in the activated methyl cycle (or S-A Met cycle) A. VB12 B. biotin C. FH4 D. VB2 E. nicotinate 烟酸

四. Answer or calculate: (7x 3= 21) 1. What is the activated reactant in each of these biosyntheses? a) Sphingomyelin 鞘磷脂 from ceramide 神经酰胺 b) Met from homocysteine 高半胱氨酸 c) Phosphatidyl choline 磷脂酰胆碱 from phosphatidyl ethanolamine 乙醇胺 d) IPP from 5-pyrophosphomevalonate 焦磷酸甲羟戊酸 e) The synthesis of phosphoribosylamine 磷酸核糖胺 f) Ser from Gly g) Glycogen n+1 from glycogen n 2. In purine biosynthesis, Asp becomes linked to the carboxylate group of an intermediate. In the next reaction, the carbon skeleton of the Asp unit leaves in the form of fumurate 延 胡索酸. These reactions are reminiscent 重演 of a similar pair of reactions in the degradation of AA. Which ones? 3. Currency exchange. For a proton-motive force of 0.2V (matrix negative), what is the maximum [ATP]/[ADP][Pi] ratio compatible with ATP synthesis? Calculate this ratio three times, assuming that the number of protons translocated per ATP formed is 2, 3, and 4 and that the temperature is 250C.

2003 TEST PAPER (1) 答案
1. hyperuricemia 高尿酸血 血液中存在异常高含量的尿酸,可能由嘌呤合成增加引起。 高尿酸血与痛 风症相关。 2. LDL receptor 低密度脂蛋白受体 一种与载脂蛋白 B 相结合的细胞表面受体。 可使 LDL 从血浆(发生细 胞)内化,以调节胆固醇和 LDL 的合成从而调节血浆胆固醇的浓度。 3. pyruvate dehydrogenase complex 丙酮酸脱氢酶复合体 催化丙酮酸、NAD+和辅酶 A 之间反应形成乙 酰辅酶 A、NADH 和 CO2(整个反应)的酶复合体。包含三个活性酶和五个辅酶。 4. glucokinase (in liver) 葡萄糖激酶 哺乳动物肝赃中特有的一种己糖激酶的同工酶,它与底物葡萄糖的 亲和力很底,故只有当血液中葡萄糖浓度很高时它才能与之反应;同时,它不受高水平 G-6-P 的抑制, 故可大量地吸纳血液中(过多)的葡萄糖。 5. malate-aspartate shuttle 苹果酸-天冬氨酸穿梭 可将还原型对应物(NADH 中的电子)从从肝脏等组织的 细胞质转运到线粒体内。 6. PRPP 磷酸核糖焦磷 生物合成多种含氮环状化合物极其衍生物中必不可少的反应物,特别在合成组 氨酸、核苷酸中的嘌呤和嘧啶时。 7. substrate cycle 底物循环 反应物和产物在两个酶的作用下的互变,如:F-6-P 与 F-1,6-P 的互变。它的存 在提高了调控的敏感性. 8. ―macro CoA‖ 巨‖辅酶 A‖; 指 ACP(酰基载体蛋白),它含有与辅酶 A 相同的磷酸泛酰巯基乙胺头部(与 脂肪酸合成过程中各中间物连接);与此部分相连接的是‖巨大‖的由 77 个氨基酸残基构成的一条多肽 链。 9. urea cycle 尿素循环; 一组循环反应, 其中由氨基酸代谢产物两个氨基和一个二氧化碳生成一个尿素 排出体外。 10.UDP- glucose 尿苷二磷酸葡萄糖; 一种核苷二磷酸糖,作为糖原合成(和其它许多合成)反应中葡萄糖 基的供体。

2003 TEST PAPER (2) 答案
二. 1. NAD+,FAD,CoA, NDP, methylation, RNA, DNA 2. B1, pyruvate DH complex, α -KG DH complex, transketolase 3. 高度还原,不含水 4. 酮脂酰-ACP 还原酶, 琥珀酸 DH; 羟脂酰-ACP 脱水酶, 延胡索酸酶; 烯脂酰-ACP 还原酶, 苹果酸 DH 5. 高半胱氨酸甲基化形成甲硫氨酸, L-甲基丙二酰 CoA 分子内重排生成琥珀酰 CoA, 高半胱氨酸甲基转移 酶, 甲基丙二酰 CoA 变位酶 6. 柠檬酸循环形成柠檬酸, 糖异生形成葡萄糖, 转氨形成天冬氨酸, 脱羧形成丙酮酸 7. UDP-葡萄糖, CDP-DAG, 磷酸化, 核苷三磷酸, 羟基 8. MVA, 3-HMG CoA, 3-HMG CoA 还原酶 9. 四氢叶酸, 蝶啶, 对氨基苯甲酸, 谷氨酸, S-腺苷甲硫氨酸, 二氧化碳, 生物素 10. G-6-P DH, LDL 受体, 内因子, 分枝酮酸 DH, NADH-Q 还原酶 11. 生物素羧基载体蛋白, 生物素羧化酶, 转羧酶 12. 磷酸化, 腺苷酸化, 糖原磷酸化酶, Gln 合成酶, Ser, Tyr 三. 1. ABCDE 2. ABCD 3. BCE 4. AC 5. ABCDE 6. A 7. ADE 8. AC

四. 1. a)CDP 胆碱 b)甲基四氢叶酸 c)S-腺苷甲硫氨酸 d)ATP e)PRPP f)亚甲基四氢叶酸 g)UDP-葡萄糖 2. 在尿素循环中: 瓜氨酸→精氨琥珀酸→精氨酸 3. 移位 2, 3, 4 个质子获得的自由能(△G = -nF△E=-46△E)分别为-9.23, .-13.8, -18.5 (kcal/mol), 合成 1 mol ATP 消耗-7.3 kcal, 分别剩余-1.93, -6.5, -11.2 kcal。 这些能量驱动合成 ATP (根据△G = 0’ 1.4 4.8 8.2 △G +1.364 log k’)直到 k’([ATP]/[ADP][Pi])值达 10 , 10 , 10 ; 分离的合成 ATP 的线粒体悬浮液中 此(实际)比值大于 104., 故每合成 1 个 ATP 移位的质子数至少为 3。